Nitric-oxide enriched plasma-activated water inactivates 229E coronavirus and alters antiviral response genes in human lung host cells

The ongoing pandemic caused by the novel coronavirus, SARS-CoV-2, is influencing global health. Moreover, there is a major threat of future coronaviruses affecting the entire world in a similar, or even more dreadful, manner. Therefore, effective and biocompatible therapeutic options against coronaviruses are urgently needed. To address this challenge, medical specialists require a well-informed and safe approach to treating human coronaviruses (HCoVs). Herein, an environmental friendly approach for viral inactivation, based on plasma technology, was considered. A microwave plasma system was employed for the generation of the high amount of gaseous nitric oxide to prepare nitric oxide enriched plasma-activated water (NO-PAW), the effects of which on coronaviruses, have not been reported to date. To determine these effects, alpha-HCoV-229E was used in an experimental model. We found that NO-PAW treatment effectively inhibited coronavirus infection in host lung cells, visualized by evaluating the cytopathic effect and expression level of spike proteins. Interestingly, NO-PAW showed minimal toxicity towards lung host cells, suggesting its potential for therapeutic application. Moreover, this new approach resulted in viral inactivation and greatly improved the gene levels involved in host antiviral responses. Together, our findings provide evidence of an initiation point for further progress toward the clinical development of antiviral treatments, including such coronaviruses. © 2022 The Authors

Nitric-oxide enriched plasma-activated water inactivates 229E coronavirus and alters antiviral response genes in human lung host cells

The ongoing pandemic caused by the novel comnavirus, SARS-CoV-2, is influencing global health. Moreover, there is a major threat of future coronaviruses affecting the entire world in a similar, or even more dreadful, manner. Therefore, effective and biocompatible therapeutic options against comnaviruses are urgently needed. To address this challenge, medical specialists require a well-informed and safe approach to treating human coronaviruses (HCoVs). Herein, an environmental friendly approach for viral inactivation, based on plasma technology, was considered. A microwave plasma system was employed for the generation of the high amount of gaseous nitric oxide to prepare nitric oxide enriched plasma-activated water (NO-PAW), the effects of which on coronaviruses, have not been reported to date. To determine these effects, alpha-HCoV-229E was used in an experimental model. We found that NO-PAW treatment effectively inhibited comnavirus infection in host lung cells, visualized by evaluating the cytopathic effect and expression level of spike proteins. Interestingly, NO-PAW showed minimal toxicity towards lung host cells, suggesting its potential for therapeutic application. Moreover, this new approach resulted in viral inactivation and greatly improved the gene levels involved in host antiviral responses. Together, our findings provide evidence of an initiation point for further progress toward the clinical development of antiviral treatments, including such comnaviruses.

Plasma bioscience and medicines

The nonthermal atmospheric biocompatible plasma, also called cold plasma, is the fourth state of matter, is a partially ionized gas consisting cocktail of gas’s molecules, free radicals, ions, electrons, and physical components such as photons, electric field and some heat. It has been successfully used in the biomedical, agricultural food safety, environment applications including industrial application for the processing of materials and etc. for not mentioned here. The recent discovery of its efficacy in sterilization of microorganisms has trigged a large quantity of research in the biomedical field. Here we review configurations and electrode layouts of typical plasma device for applications to biomedical such as cancer treatment and virus inactivation technology. Cocktail of reactive oxygen and nitrogen species (RONS) would be efficient and effective to several biomedical applications such as inactivation of drug-resistant bacteria, cancer treatment, inactivation of viruses, skin and dental applications. This detailed review provides an outline of typical plasma sources, their physical and chemical (RONS) characteristics based on their diagnostic methods, including cancer treatment strategies and inactivation of viruses. This review also emphasizing on strategises to control and inactivation of SARS-COV2 (COVID19) and rejuvenate lung cells. Plasma bioscience and medicine technologies will deliver a new model of therapeutic clinical systems along with sustainable application to environmental issues. © 2021, Korean Vacuum Society. All rights reserved.

Promising iron(II) complexes of curcumins: designing, density functional theory, and molecular docking

Curcumin is the key compound of turmeric and was identified as diferuloylmethane. It can be administered through various routes like orally and inhalation. It is hydrophobic, and the bioavailability for oral administration of curcumin is low. It is an important biological promising compound and used for the treatment of patients' suffering from atherosclerosis, colon cancer, hypercholesteremia, inflammatory bowel disease, pancreatic cancer, and so forth. The work aims to design the iron(II) complexes of curcumin and its derivatives. The designed complexes were studied by the density functional theory (DFT) approach for their stability and were investigated based on IR spectra using Gaussian 9.0. Further, curcumin and the iron (II) complexes were explored for their biological potency as inhibitors against the main protease of SARS-CoV-2, as well as for antimicrobial and antioxidant activities using molecular docking with the iGemDock. Based on the results, it has been observed that among the iron(II) complexes of curcumin, C1 showed promising biological activities against tyrosinase kinase and the main protease of SARS-CoV-2 as well as satisfactory action against Escherichia coli. © 2021 John Wiley & Sons, Ltd.